Mentored by Isabel Pastor del Campo and Lukas Kühnemann, of the Small Biosystems Lab. The abstract, as submitted:
Most of what we know about molecules comes out of averages of over 10²³ of them, smoothing out the complications, individual jumps, pauses and failures that make molecular behaviour unpredictable in specifics. Single-molecule force spectroscopy lets us do thermodynamics one object at a time, pulling apart a DNA hairpin while force and distance are recorded. With this talk I'll follow a measurement from the bench to the number. How optical tweezers operate, how they are built, and how an irreversible pull's force-distance curve becomes a free energy measurement through fluctuation theorems. Based on a six-week research stay in the group of Fèlix Ritort, Universitat de Barcelona, where I learned the instrument, built the chambers these experiments run in, and got to operate the optical tweezers.